Single-cell sequencing reveals karyotype heterogeneity in murine and human malignancies

Authors: Bakker, Bjorn and Taudt, Aaron and Belderbos, Mirjam E. and Porubsky, David and Spierings, Diana C. J. and de Jong, Tristan V. and Halsema, Nancy and Kazemier, Hinke G. and Hoekstra-Wakker, Karina and Bradley, Allan and de Bont, Eveline S. J. M. and van den Berg, Anke and Guryev, Victor and Lansdorp, Peter M. and Colomé-Tatché, Maria and Foijer, Floris Year: 2016 Journal: Genome Biology

Abstract

Chromosome instability leads to aneuploidy, a state in which cells have abnormal numbers of chromosomes, and is found in two out of three cancers. In a chromosomal instable p53 deficient mouse model with accelerated lymphomagenesis, we previously observed whole chromosome copy number changes affecting all lymphoma cells. This suggests that chromosome instability is somehow suppressed in the aneuploid lymphomas or that selection for frequently lost/gained chromosomes out-competes the CIN-imposed mis-segregation.

Notes

Extracted Concepts

• AneuFinder enables automated quantification of copy number heterogeneity
• Bulk sequencing masks intratumoral copy number heterogeneity
• Mps1 truncation-induced CIN generates convergent recurrent chromosome gains
• Single cells show unique chromosome copy numbers beyond clonal karyotype
• Single-cell sequencing reveals karyotype heterogeneity in lymphomas
• scWGS platform detects copy number variants via shallow sequencing