Proteins encoded by genes on duplicated chromosomes in aneuploid yeast strains show increased abundance of approximately twofold compared to wild-type cells. This correlation is consistent across all 12 disomic strains examined, with protein levels increasing proportionally to gene dosage on the duplicated chromosomes. [@dephoure_quantitative_2014]
Definitions
- aneuploidaneuploidy
- protein abundance
- gene dosage
- disomic strains
- duplicated chromosomes
- proteome
- aneuploid yeast
- wild-type cells
Synthesis
Studies using quantitative proteomics approaches such as SILAC and liquid chromatography-tandem mass spectrometry have established that aneuploidy causes a proportional increase in protein abundance from duplicated chromosomes, with proteins encoded on extra chromosomes showing approximately twofold elevation compared to those on normal chromosomes. This direct quantitative relationship between gene dosage and protein stoichiometry demonstrates high fidelity tracking, where chromosomal-scale genomic perturbations translate into corresponding proteomic changes that drive the cellular phenotypes observed in aneuploid strains, including cell cycle defects and altered glucose uptake. The strength of this correlation is evident when protein abundance ratios are sorted by chromosomal position, revealing consistent elevation patterns for proteins from duplicated chromosomes. However, growth medium conditions may significantly influence proteome composition in disomic strains, suggesting that environmental factors could modulate the extent or impact of these dosage-driven protein imbalances.
Related
- Growth medium conditions affect aneuploid strain proteome composition
- Quantitative proteomics measures 70-80% of yeast open reading frames
- Extra chromosome genes drive aneuploid phenotypes through imbalanced protein composition
- Protein levels correlate with chromosomal position in aneuploid strains